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    型號:PRE001C-50ul
    品牌:PREGENE
    原產地:美國
    類別:化工 / 生物化學品
    標籤︰內參抗體 , 標籤抗體 , 斑馬魚抗體
    單價: ¥600 / 件
    最少訂量:1 件
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    會員信息

    詳細信息

    北京普瑞金科技有限公司

    免費會員北京市东城区
    即時通訊:最後上線︰2014/03/04

    產品描述

    Flag-Tag Mouse Monoclonal Antibody(2C5)

    Catalog

    PRE001C

    PRE001F

    Tel010-57129029, 18610667573

    Quantity

    50μL

    100μL

    For research use only.

    Applications

    Species Cross-Reactivity

    Molecular Weight

    Isotype

    WB, IP, IF

    N/A

    N/A

    IgG1

    Storage Buffer & Condition:  PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.      Store at -20°C. Do not aliquot the antibody.

    Recommended dilutions: WB: 1:5,000             IP: 1:200             IF: 1:2000

    Optimal dilutions should be determined by the end user.

    Specificity: The Flag tag antibody can recognize C-terminal, internal, and N-terminal Flag-tag fusion proteins.

    Background:The DYKDDDDK peptide (Flag-tag) is a small component of an epitope which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. It has been used extensively as a general epitope tag in expression vectors. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.A Flag-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a Flag-tag to this protein allows one to follow the protein with an antibody against the Flag sequence.

    1ug Flag fusion protein+ Primary antibody dilution at 1:10,000

    IP antibody use5ug Flag Mouse IgG1 per ml LysateWB 1:5000

    1untransfected 293 cell lysate

    2transfected 293 cell lysate with Flag-tag fusion protein

    3IP (transfected 293+ normal Mouse IgG+Protein G agarose)

    4IP (transfected 293+anti- Flag mAb+ Protein G agarose)

    5IP (transfected 293+Protein G)

    6Recombinant protein (E.coli)

     

    IF analysis of 293 cells transfected with a Flag-tag protein, using anti-FlagTag Mouse mAb at a 1:2000 dilution (blue DAPI, red anti-Flag)

     


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    北京普瑞金科技有限公司

    北京市海澱區知春路51號5層5411室

    電話︰
    86-010-57129029
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    86-010-63867923
    聯繫人︰
    李經理 (銷售經理)
    手機︰
    57129029
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