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西班牙瓊脂糖Biowest Agarose

產品介紹：

中文全名：瓊脂糖
英文全名：Agarose
別名：瓊膠糖；瓊膠素

品牌：Biowest

產地：西班牙

規格：100g
分子式：C12H18O9
分子量：306.26
外觀：白色或微黃色粉末
說明：DNA、脂蛋白和免疫電泳用；生化研究免疫擴散等的底物
特性：純度：總雜質：≤1.0% 灰份，≤10% 水分
儲存條件：室溫

產品詳情：

瓊脂（Agar），又名瓊膠、菜燕、凍粉，是一類從石花菜及其它紅藻類（Rhodophyceae）植物提取出來的藻膠（phycocottoid），在我國及日本已有三百多年（1658年）的曆史。瓊脂糖Agarose，縮寫為AG，是瓊脂中不帶電荷的中性組成成份，也譯為瓊膠素或瓊膠糖。瓊膠糖化學結構由β-D-吡喃半乳糖(1-4)連接3,6-脫水α-L-吡喃半乳糖基單位構成.

產品用途：瓊脂、瓊脂糖因為有特殊的膠凝性質，尤其有顯著的穩固性、滯度和滯后性，並且易吸收水分，有特殊的穩定效應；已經廣氾使用於食用、醫藥、化工、紡織等領域，據不完全統計，瓊脂、瓊脂糖的用途已有1000多種，被國際上稱為“新奇的東亞產品”。在食品工業中可用於生產：水晶軟糖、定型軟糖、水產品、肉類罐頭、果汁飲料、果肉飲料、米酒飲料、乳品飲料、精品、乳品蛋糕。

近年來由於其良好的生物相容性又廣氾用於生物分離介質的生產。 

權威的西班牙瓊脂糖（Biowest Agarose）是一種電泳級別純度很高的產品，作為著名的瓊脂糖品牌，具有極佳的產品性價比，在同行中有着非常高的知名度，在同類產品中佔據着較高的市場份額，西班牙瓊脂糖（Biowest Agarose）不僅適用於蛋白電泳也可以用於其它核酸分析電泳

Biowest 技術參數：

Agarose Low EEO

Gel Strength (1.0%) (g/cm2)………………≥750

Gelling Range (1.5%) (℃)………………36 - 39

Melting Range (℃)………………………87 - 89

EEO (-mr)……………………………………≤0.15

Sulfate (%)………………………………≤0.15

DNase……………………………………None Detected

RNase……………………………………None Detected

Protease………………………………None Detected

Introduction:

Routine use agarose is ideal for everyday analysis of nucleic acids by gel electrophoresis or blotting (Northern or Southern) and is also suitable for protein applications such as Ouchterlony and radial immunodiffusion (RID).

Analysis Note :

Sulfate content- used as an indicator of purity, since sulfate is the major ionic group present.
Gel strength- the force that must be applied to a gel to cause it to fracture.
Gel point- the temperature at which an aqueous agarose solution forms a gel as it cools. Agarose solutions exhibit hysteresis in the liquid-to-gel transition - that is, their gel point is not the same as their melting temperature.
Electroendosmosis (EEO)- a movement of liquid through the gel. Anionic groups in an agarose gel are affixed to the matrix and cannot move, but dissociable counter cations can migrate toward the cathode in the matrix, giving rise to EEO. Since electrophoretic movement of biopolymers is usually toward the anode, EEO can disrupt separations because of internal convection.

Preparation of Agarose Gels for DNA separations:

Weigh out the desired amount of agarose and place in an Erlenmeyer flask with a measured amount of electrophoresis buffer, e.g. for an 0.8% gel, add 0.8 gm of agarose and 100ml of TBE Buffer (1X), to a 200 ml flask. The larger flask insures against the agarose boiling over. Dissolve the agarose in a boiling water bath or in a revolving-plate microwave oven. All the grains of agarose should be dissolved and the solution clear. Cool the solution to 60°C ( 70°C for concentrations 2% or above) and pour immediately. Allow the gel to set for one-half hour before using. Make sure to use the same electrophoresis buffer in the gel as for the running buffer.

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